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Literature summary extracted from

  • Matsui, D.; Asano, Y.
    Heterologous production of L-lysine epsilon-oxidase by directed evolution using a fusion reporter method (2015), Biosci. Biotechnol. Biochem., 79, 1473-1480 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.20 NaCl highest enzyme activity is obtained by incubation with 1.6 M NaCl, which is approximately 1.5fold higher than that of the untreated enzyme Marinomonas mediterranea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.20 expressed in Escherichia coli BL21(DE3) cells Marinomonas mediterranea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.20 0.0018
-
 L-lysine at pH and °C Marinomonas mediterranea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.20 160000
-
 gel filtration Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.20 Marinomonas mediterranea F2JXJ3
-
-
1.4.3.20 Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O 100% activity Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O 100% activity Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-ornithine + O2 + H2O 15.1% activity compared to L-lysine Marinomonas mediterranea ? + H2O2 + NH3
-
 ?
1.4.3.20 L-ornithine + O2 + H2O 15.1% activity compared to L-lysine Marinomonas mediterranea NBRC 103028 ? + H2O2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.20 homodimer 2 * 97000, SDS-PAGE Marinomonas mediterranea

Synonyms

EC Number Synonyms Comment Organism
1.4.3.20 L-lysine epsilon-oxidase
-
 Marinomonas mediterranea
1.4.3.20 LodA
-
 Marinomonas mediterranea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.20 55
-
-
 Marinomonas mediterranea